Indonesia, as an archipelago rich in marine resources, holds immense potential in the maritime sector. One of its flagship commodities, grouper fish—specifically the tiger grouper variety—has successfully entered international markets. However, grouper aquaculture faces significant challenges, one of which is the threat of diseases caused by viruses.
One of the most lethal viruses is Viral Nervous Necrosis (VNN). This virus attacks groupers from the larval stage to juveniles, causing mortality rates of up to 100%. Transmission can occur vertically (from parent to offspring) or horizontally (between fish in the same population).
To gain deeper insights into VNN, FPK Unair students conducted a Fieldwork Practice (PKL) at the Brackish Water Aquaculture Development Center (BPBAP) in Situbondo. This activity took place over a month, from January 18 to February 18, 2016, at the facility located on Jalan Raya Pecaron, Situbondo Regency, East Java.
The fieldwork adopted a descriptive method, collecting data through observation, interviews, documentation, and active participation. One of the key steps in this program was diagnosing VNN using the Polymerase Chain Reaction (PCR) method. The process involved:
- Sample reception
- DNA extraction
- Preparation of 2% agarose gel
- Electrophoresis
- Reading electrophoresis results
The PCR method provides accurate virus detection, enabling effective prevention and control measures to be implemented.
For those interested in exploring this topic further, the Faculty of Fisheries and Marine Sciences at Unair offers a collection of literature available at the FPK Unair Reading Room. Let’s optimize our knowledge for the advancement of Indonesia’s marine aquaculture!
